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How We Can Support the Drug Development Pipeline

DME TOX

It is estimated that nearly 50% of drugs fail due to metabolic instability, poor distribution, metabolic non responders and undesirable side effects. Source BioScience offers Pharma/ Biotech clients SNP genotyping using ABI and Roche AmpliChip for P450 metabolism analysis.

Source BioScience also offers a range of tests for mutation analysis to determine response to drugs with genetic testing recommended on the label. For further information, please go to mutation analysis. 

Assay Technology

The GoldenGate assay is used to interrogate 384 - 1536 SNPs simultaneously by using allele specific extension and ligation. The high level of multiplexing minimises the time and reagents required for processing. Researchers can select SNP loci of choice to form customer panels for targeted genotyping or an expanding selection of standard panels is also available including panels for cancer research.
Illumina goldengate custom panels

Roche AmpliChip®amplichip.PNGroche.PNG

The Roche AmpliChip® platform is the first pharmacogenetic microarray-based test approved for clinical use.

The AmpliChip® CYP450 test identifies a patient's genotype by analysing CYP2D6 and CYP2C19, two genes in the cytochrome P450 system that can greatly influence drug metabolism. These two genes code for enzymes that metabolise many antidepressants, antipsychotics, and ADHD drugs, as well as other medications. The AmpliChip® CYP450 test can help physicians adjust dosing and select drugs by predicting a phenotype based on a genotype so that patient treatment can be individualised to get the best possible therapeutic results. It can also be used to stratify patients in clinical trials.

Source BioScience is a preferred supplier for Roche AmpliChip™ in the UK.
Why use Source BioScience?

  • Quality: Rapid turnaround and accurate results. All data is sent in strictest confidence to you on CD-ROM, e-mail or web server. We are also GLP,GCP ,CPA accredited and a UK preferred service supplier for ABI and Roche.
  • Pricing: Our high throughput reduces unit labour and capital costs meaning we can offer exceptional value for money. Furthermore, savings are made by bulk purchase of reagents.
  • Personal Touch: We can also design, validate and genotype user-defined SNPs.
  • Commitment to improvement: We are continuously introducing new technologies to reduce costs, shorten timelines and reduce consumables including amounts of your DNA.

Applied Biosystems TaqMan® SNP Genotyping

The TaqMan® platform is suitable for projects of 10s of SNPs and 1000s of Samples. For higher throughput projects customers should consider SNPlex.

Platforms

  • TaqMan assay for medium throughput applications.
  • MATRIX PlateMatePlus for high throughput liquid handling.
  • KBiosystems Super Duncan thermal cycler.
  • ABI PRISM 7900HT Sequence Detection Systems permit high throughput data collection.

For further information on SNP genotyping assays available for drug development and metabolism, please click here.

Applied Biosystems SNPlex™ 48-plex SNP genotyping system

Description

Single Nucleotide Polymorphisms are bi-allelic, single base-pair changes between the DNA sequences of an individual. They can exist within a population at any frequency although those with a frequency above 20% are generally most useful in genetic mapping studies. SNPs are found throughout the genome averaging one SNP every 500 to 1000bp. They can affect translation and transcription. In some circumstances they lead to profound disease whereas elsewhere will have no obvious effect. SNPs are also important in predicting response to therapeutics. Using SNP mutation analysis can enrich patient populations for clinical trials providing the drug development team with meaningful data.
The SNPlex ™ Genotyping System uses an Applied Biosystems oligonucleotide ligation assay (OLA) combined with multiplex PCR technology to achieve allelic discrimination and target amplification.
Source BioScience is an approved service provider for Applied Biosystems SNPlex system

Application

The special virtues of SNPs for genetic analysis are their ease of discovery and assay, and their ease of analysis. Their high density in the human genome and their effects, in some cases, on transcription and translation make SNPs valuable for association studies. The SNPlex platform is ideal for use in screening candidate genes for associations or for confirming genome-wide associations in follow-up populations.
SNPlex studies are of the order of 100s of SNPs genotyped in 1000s of DNA samples. For lower throughput projects customers should consider TaqMan.

Platforms

  • MATRIX PlateMatePlus for high throughput liquid handling.
  • SNPlex 48-plex SNP genotyping system for simultaneously assay of large numbers of SNPs.
  • Applied Biosystems GeneAmp PCR system 9700, and MJ Research PTC-225 tetrad - PCR.
  • Applied Biosystems 3730XL DNA analyser.
  • GeneMapper v4.0 from Applied Biosystems.

Use Source BioScience because we can provide:

  • Quality: Rapid turnaround and accurate results. All data is sent in strictest confidence to you on CD-ROM, e-mail or web server.
  • Pricing: Our high throughput reduces unit labour and capital costs meaning we can offer exceptional value for money. Furthermore, savings are made by bulk purchase of reagents and discount deals with suppliers.
  • Commitment to improvement: We constantly review and implement new technologies to reduce costs, shorten timelines and reduce consumables including amounts of your DNA.

Cross Reactivity Testing

PBADME.PNGSource BioScience offers cross reactivity testing to ascertain adverse reactions in a range of tissues comprising FDA and FDA/European lists. We have a dedicated project team who can help to develop customised study designs to meet project requirements and deadlines. We have significant experience in a range of small molecules and compounds including phosphorylated antibody studies for FDA submissions. Images produced after staining can be viewed via the web using a secure web browser. Expert pathology review of sections is also offered.

To discuss further with one of our team, please email us at  pharma

Source BioScience offers a range of tests for mutation analysis to determine response to drugs with genetic testing recommended on the label. For further information, please click here.

MicroRNAs

Source BioScience can provide a miRNA quantitation service with a simple two-step protocol that requires only reverse transcription with a miRNA-specific primer, followed by real-time PCR with TaqMan® probes.

Microfluidic card formats

Source BioScience offers GPCR Arrays in microfluidic card formats for three species: human, mouse and rat. The GPCR targets selected fall into approximately 50 subfamilies associated with drug targets or disease.

PhenoFiler™

PhenoFiler™ is a novel platform which combines high throughput target profiling with proprietary bioinformatics analysis and with high-content siRNA knockdown phenotypic screening in cell cultures. SourceBioScience can provide these services to support ADME Tox as well as other gene analysis studies.

  • Glutathione s-transferases (GST)
  • Sulfonyl transferases (SULT)
  • N-acetyl transferase type 2 (NAT2)
  • Thiopurine methyltranferases (TPMT)
  • Uridine diphosphate-glucoronyl transferases (UGT)

Please download the pdf leaflet for further information on ADME TOX.